Journal: Frontiers in Cellular Neuroscience

Article Title: GABAergic synaptic components are largely preserved across human and mouse neuronal models

doi: 10.3389/fncel.2025.1588894

Figure Lengend Snippet: Morphological characterization of GABAergic neurons. (A–D) Scatter-plot showing mean soma areas (A) , dendritic thickness (B) , dendritic lengths (C) , and VGAT synapse puncta (D) . (E) Representative images of neuronal morphology showing immunostaining for MAP2 (color coded) and VGAT (black). Scale bars, 40 μm. Data shown as mean ± SEM. Kruskal-Wallis test: * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, and **** p ≤ 0.0001.

Article Snippet: Specifically, primary antibodies dilutions were as follows: chicken anti-microtubule-associated protein 2 (MAP2) (1:2000, Millipore), rabbit anti-vesicular GABA transporter (VGAT) (1:1000, Synaptic Systems).

Techniques: Immunostaining